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1992 3
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Page 1
Water-Based Solid-Phase Peptide Synthesis without Hydroxy Side Chain Protection.
Hojo K, Manabe Y, Uda T, Tsuda Y. Hojo K, et al. J Org Chem. 2022 Sep 2;87(17):11362-11368. doi: 10.1021/acs.joc.2c00828. Epub 2022 Aug 15. J Org Chem. 2022. PMID: 35969667
We also succeeded in synthesizing a difficult peptide sequence, acyl carrier protein (65-74) peptide, by aqueous SPPS without hydroxy or carboxamide side chain protection. ...
We also succeeded in synthesizing a difficult peptide sequence, acyl carrier protein (65-74) peptide, by …
Peptide synthesis on Sepharose beads.
Tegge W, Frank R. Tegge W, et al. J Pept Res. 1997 Apr;49(4):355-62. doi: 10.1111/j.1399-3011.1997.tb01137.x. J Pept Res. 1997. PMID: 9176820
The amide linker p-[(R1S)-alpha-[1-(9H-fluoren-9-yl)-methoxyformamido]- 2, 4-dimethoxybenzyl]phenoxyacetic acid was incorporated and the acyl carrier protein sequence 65-74 was synthesized manually on this support by the Fmoc procedure un …
The amide linker p-[(R1S)-alpha-[1-(9H-fluoren-9-yl)-methoxyformamido]- 2, 4-dimethoxybenzyl]phenoxyacetic acid was incorporated and …
An endogenous proteinacious inhibitor in porcine liver for S-adenosyl-L-methionine dependent methylation reactions: identification as oligosaccharide-linked acyl carrier protein.
Seo DW, Moon HI, Han JW, Hong SY, Lee HY, Kim S, Paik WK, Lee HW. Seo DW, et al. Int J Biochem Cell Biol. 2000 Apr;32(4):455-64. doi: 10.1016/s1357-2725(99)00144-2. Int J Biochem Cell Biol. 2000. PMID: 10762071
A 7400 Da polypeptide fragment of the purified inhibitor was sequenced by matrix-associated laser desorption ionization; time-of-flight MS, and was found to be identical with the known sequence of spinach acyl carrier protein (ACP). Although the remainder of …
A 7400 Da polypeptide fragment of the purified inhibitor was sequenced by matrix-associated laser desorption ionization; time-of-flight MS, …
Sequence-assisted peptide synthesis (SAPS).
Due Larsen B, Holm A. Due Larsen B, et al. J Pept Res. 1998 Dec;52(6):470-6. doi: 10.1111/j.1399-3011.1998.tb01251.x. J Pept Res. 1998. PMID: 9924991
A mixed pre-sequence, (Lys(Boc)Glu(tBu))3, has a similar favorable effect on the synthetic results, but the positive effect seems confined to a rather narrow framework of amino acids and side-chain protecting groups in the pre-sequence as discussed in the article. Among ot …
A mixed pre-sequence, (Lys(Boc)Glu(tBu))3, has a similar favorable effect on the synthetic results, but the positive effect seems confined t …
Solid-phase peptide synthesis using microwave irradiation.
Murray JK, Aral J, Miranda LP. Murray JK, et al. Methods Mol Biol. 2011;716:73-88. doi: 10.1007/978-1-61779-012-6_5. Methods Mol Biol. 2011. PMID: 21318901
These microwave-assisted peptide synthesis procedures have been used to rapidly prepare a "difficult" peptide sequence from the acyl carrier protein, ACP(65-74), in less than 3 h and the reduced, linear precursor to human hepcidin, in high initi …
These microwave-assisted peptide synthesis procedures have been used to rapidly prepare a "difficult" peptide sequence from the acyl …
Accelerated chemical synthesis of peptides and small proteins.
Miranda LP, Alewood PF. Miranda LP, et al. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1181-6. doi: 10.1073/pnas.96.4.1181. Proc Natl Acad Sci U S A. 1999. PMID: 9989998 Free PMC article.
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recombinant protein overexpression and is widely used in structural biology, immunology, protein engineering, and biomedical research. ...This rapid coupling chemistry was …
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recombinant protein overexpression and …
Microwave-assisted solid-phase peptide synthesis based on the Fmoc protecting group strategy (CEM).
Vanier GS. Vanier GS. Methods Mol Biol. 2013;1047:235-49. doi: 10.1007/978-1-62703-544-6_17. Methods Mol Biol. 2013. PMID: 23943491
Microwave technology significantly reduces the synthesis time while also improving the quality of the peptides produced. Microwave energy allows most amino acid couplings to be completed in just 5 min. The Fmoc removal can also be accelerated in the microwave decreasing th …
Microwave technology significantly reduces the synthesis time while also improving the quality of the peptides produced. Microwave energy al …
Ethyl 2-cyano-2-(2-nitrobenzenesulfonyloxyimino)acetate (o-NosylOXY): a recyclable coupling reagent for racemization-free synthesis of peptide, amide, hydroxamate, and ester.
Dev D, Palakurthy NB, Thalluri K, Chandra J, Mandal B. Dev D, et al. J Org Chem. 2014 Jun 20;79(12):5420-31. doi: 10.1021/jo500292m. Epub 2014 Jun 4. J Org Chem. 2014. PMID: 24849944
The synthesis of the difficult sequences, for example, the islet amyloid polypeptide (22-27) fragment (with a C-terminal Gly, H-Asn-Phe-Gly-Ala-Ile-Leu-Gly-NH2) and acyl carrier protein (65-74) fragment (H-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly …
The synthesis of the difficult sequences, for example, the islet amyloid polypeptide (22-27) fragment (with a C-terminal Gly, H-Asn-Phe-Gly- …
Ion-spray tandem mass spectrometry in peptide synthesis: structural characterization of minor by-products in the synthesis of ACP(65-74).
Schnölzer M, Jones A, Alewood PF, Kent SB. Schnölzer M, et al. Anal Biochem. 1992 Aug 1;204(2):335-43. doi: 10.1016/0003-2697(92)90249-7. Anal Biochem. 1992. PMID: 1443533
Ion-spray triple quadrupole mass spectrometry was used to investigate the products from the solid phase synthesis of the decapeptide (H)-Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-(OH) [acyl carrier protein(65-74)]. The target sequence was assemble …
Ion-spray triple quadrupole mass spectrometry was used to investigate the products from the solid phase synthesis of the decapeptide (H)-Val …
17 results